Phytochemical prospection, identification and evaluation of microbiological activity of secondary metabolites of Annona mucosa Jacq
Keywords:
Phytochemical prospection, Alkaloids, Flavonoids, Microbiological activity, Mucosal Annona.Abstract
The present work describes the results obtained for the phytochemical and microbiological studies of the 70% hydroethanolic extract, its fractions and metabolites isolated from the leaves of the Biribazeiro (Annona mucosa Jacq.). Initially, the preparation of the hydroethanolic extract (70%) of the leaves of the plant was carried out and a phytochemical screening was carried out in search of the secondary metabolites of the study. Tests confirmed the presence of tannins, flavonols, flavanones, flavananols, xanthones, leucoanthocyanidins, anthocyanidins, free steroids,
saponins and alkaloids. Then, the organic fractionation of the crude extract was performed, and through thin layer chromatography of the chloroform fraction, the separation of mixtures of alkaloids and flavonoids that were identified by hyphenated techniques of LC-ESI-IT-MS/MS and FIA-ESI-IT-MSn, such as the alkaloid romucosin and the anthocyanidin delphinidin-3-O-β-glucopyranoside, was performed. In addition to the isolation and identification of secondary metabolites, this work presents results for microbiological evaluation through the technique of diffusion in solid well drilling medium with samples of 70% crude hydroethanolic extract as well as isolated fractions and metabolites. Through these assays, satisfactory results were obtained against the bacterium Staphylococcus aureaus ATCC, the fungus Candida albicans and the clinical isolate of Salmonella spp, attributing these activities to the presence of the identified alkaloids and flavonoids. The phytochemical results obtained corroborate the botanical identification of the studied species Annona mucosa Jacq. as belonging to the Annonaceae family, due to the presence of substances that are commonly found in plants belonging to this family and through microbiological evaluation as an important source of chemical substances in the production of new medicines.
