Comparative study of methodologies for the determination of of proteins and lipids in quinoa
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Abstract
The centesimal analysis of grains is performed with the crushed sample as a way to minimize time and reduce costs with reagents. However, milling is an expensive task when there are a large number of samples. Thus, this work evaluated the need for grinding quinoa grains for the determination of protein and lipids by the semi-micro Kjeldahl method, using concentrated sulfuric acid and a temperature of 450 °C, and by the methodology using petroleum ether in an automatic extractor (lipids ). There was no statistically significant difference between whole and ground grain samples. Thus, it is recommended that, for protein and lipid analysis with these methodologies, it is not necessary to grind the grains.
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Copyright (c) 2023 Andréia Alves Rosa, Juliana Evangelista da Silva Rocha, Artur Guerra Rosa, Carlos Roberto Spehar