PRODUCTION AND CHARACTERIZATION OF CARBOXYMETHYL CELLULASE BY SUBMERGED FERMENTATION OF STACHYLIDIUM BICOLOR

Abstract

Microorganisms that are capable of degrading lignocellulolytic materials can produce extracellular cellulase complexes. Fungal are good producers of cellulolytic complex, since these microorganism sources have a high capacity of multiplication and production of various enzymes, enabling industrial production. At this work, we researched the production of the enzyme carboxymethyl cellulase (CMCase) by the fungus Stachylidium bicolor. The pH and temperature optimum were studied by Response surface methodology with different temperatures (30, 50, 70ºC) at different pHs, ranging from 2 to 6. The effect of substances (sodium chloride, sodium benzoate and monosodium phosphate) was studied as stabilizer CMCase using a simple-centroid mixture design. The enzyme showed optimum pH and temperature at 4.0 and 50 ^°C, respectively. Monosodium phosphate showed higher and positive EP, suggesting that the mixture with a higher proportion of this component provides a higher enzymatic activity.

DOI: https://doi.org/10.56238/sevened2024.037-096