Insecticidal and juvenilizing activity tests of Euphorbia tirucalli Latex on Aedes aegypti Larvae. Micropropagation and new potential for biological activity

Abstract

Euphorbia tirucalli is a succulent species and its latex is employed with several uses, amongst which vector control, as mosquitoes, at Africa and Asia. Due to latex’s citotoxicity, by the presence of diterpenoids, in this study we analyzed insecticide and juvenilizing effects evoked by aqueous fraction from latex obtained from explants micropropagated with 6-Benzylaminopurin (6-BA) (1.0 mg/L) besides 6-Benzylaminopurin (6-BA) (1.0 mg/L) with Norbixin, both under white and red  light fluences, during 20 days. Latex was extracted in water from stems submitted to ultrasonic method from all explants to be tested upon development of Aedes aegypti larvae. Neither insecticide nor juvenilizing  activities were noticed to all aqueous fraction of raw latex and explants tested at 100 microlitres. These results are distinct of classic literature. After qualitative anatomic analysis of explants comparing to garden control, new micropropagations with same vegetal regulator and antioxidant will be performed, but with more during time of red light irradiance at cultive. Besides it, higher concentrations of those aqueous latex extracts will be performed, due to high prevalence of Dengue in Rio de Janeiro.

DOI:https://doi.org/10.56238/sevened2024.011-003