Insecticidal and juvenilizing activity tests of Euphorbia tirucalli Latex on Aedes aegypti Larvae. Micropropagation and new potential for biological activity
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Abstract
Euphorbia tirucalli is a succulent species and its latex is employed with several uses, amongst which vector control, as mosquitoes, at Africa and Asia. Due to latex’s citotoxicity, by the presence of diterpenoids, in this study we analyzed insecticide and juvenilizing effects evoked by aqueous fraction from latex obtained from explants micropropagated with 6-Benzylaminopurin (6-BA) (1.0 mg/L) besides 6-Benzylaminopurin (6-BA) (1.0 mg/L) with Norbixin, both under white and red light fluences, during 20 days. Latex was extracted in water from stems submitted to ultrasonic method from all explants to be tested upon development of Aedes aegypti larvae. Neither insecticide nor juvenilizing activities were noticed to all aqueous fraction of raw latex and explants tested at 100 microlitres. These results are distinct of classic literature. After qualitative anatomic analysis of explants comparing to garden control, new micropropagations with same vegetal regulator and antioxidant will be performed, but with more during time of red light irradiance at cultive. Besides it, higher concentrations of those aqueous latex extracts will be performed, due to high prevalence of Dengue in Rio de Janeiro.
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Copyright (c) 2024 Márcia Cristina Braga Nunes Varricchio, Sandra Villa Gaspar, Rayssa de Macedo Rebelo, Morgana Teixeira Lima Castelo Branco, Alexandre dos Santos Pyrrho, Simone da Silva, Celso Luiz Salgueiro Lage, Paulo Sérgio Torres BriosoAuthor(s)
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Márcia Cristina Braga Nunes Varricchio
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Sandra Ávila Gaspar
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Rayssa de Macedo Rebelo
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Morgana Teixeira Lima Castelo Branco
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Alexandre dos Santos Pyrrho
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Simone da Silva
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Celso Luiz Salgueiro Lage
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Paulo Sérgio Torres Brioso