Molecular and structural characterization of cDNA from a cellulase of Moniliophthora perniciosa (Stahel) Aime & Phillips-Mora
Abstract
The basidiomycete fungus Moniliophthora perniciosa (Stahel) Aime & Phillips-Mora, is a phytopathogen, causing witches' broom disease and is known as a potential producer of cellulases enzymes. These enzymes act on the degradation of the plant cell wall and can be produced by numerous microorganisms, with filamentous fungi being the main producers. Cellulases have a wide variety of industrial applications and the production of cellulases from microbial sources is an excellent alternative for the production of cellulolytic enzymes such as endoglucanase. This work presents the expression related to a hypothetical protein of the GH45 family of the fungus Moniliophthora perniciosa, through cDNA analysis and the determination of its 3D structure by comparative modeling, presenting a high quality model, showing a GH45 with a classic structure characteristic of endoglucanases.
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Copyright (c) 2023 Cleidineia Souza de Santana, Alison Borges Vitor, Edjane Bastos Ferreira, Luiz Henrique Machado Oliveira, Jamille de Santana Melo Adorno, Sandra Aparecida de Assis, Raquel Guimarães BenevidesAuthor(s)
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Cleidineia Souza de Santana
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Alison Borges Vitor
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Edjane Bastos Ferreira
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Luiz Henrique Machado Oliveira
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Jamille de Santana Melo Adorno
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Sandra Aparecida de Assis
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Raquel Guimarães Benevides