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Molecular biology - Some techniques and applications: Literature review

Pinto MS;
Borges IR;
Fontoura LGO;
Carneiro LC;
Filho AVM

Murillo de Sousa Pinto

Isabela da Rosa Borges

Luiz Guilherme Oliveira Fontoura

Lilian Carla Carneiro

Aroldo Vieira de Moraes Filho


Keywords

Deoxyribonucleic acid
Ribonucleic acid
Polymerase Chain Reaction

Abstract

Molecular Biology is an area of Biology that is dedicated to the molecular structure of intracellular chemical and biological reactions and was established due to the study of the structure of DNA, RNA, protein synthesis and various molecular techniques. The evolution of these techniques has enabled the detection of diseases and genetic errors more quickly and effectively for diagnosis and treatment. Given this, this work uses a literature review on the use of Molecular Biology, in order to highlight its positive and negative points. Molecular study is strongly linked to the function of genes and gene products, which makes the approach to molecular biology expansive. By sequencing the genome of several organisms, it is concluded that nucleotide sequences alone are not sufficient to fully understand the functions of genes. Already, in relation to the techniques, it is known that the PCR have its techniques delineated around the Central Dogma of Molecular Biology executing in vitro processes of Transcription by Polymerase Chain Reaction. After data analysis, it is concluded that there was an evolution of Molecular Biology techniques as well as their application, so the positive points stand out in relation to the negatives.

 

DOI:https://doi.org/10.56238/uniknowindevolp-075


Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Copyright (c) 2023 Murillo de Sousa Pinto, Isabela da Rosa Borges, Luiz Guilherme Oliveira Fontoura, Lilian Carla Carneiro, Aroldo Vieira de Moraes Filho

Author(s)

  • Murillo de Sousa Pinto
  • Isabela da Rosa Borges
  • Luiz Guilherme Oliveira Fontoura
  • Lilian Carla Carneiro
  • Aroldo Vieira de Moraes Filho