Identification of microorganisms present in an antineoplastic manipulation environment in a tertiary hospital in Brasília

Abstract

There is a growing concern about good practices for compounding sterile drugs in hospitals, due to the compounding room itself and the specific public that will use these products. The identification of the cleanroom microbiota in the Antineoplastics Compounding Unit (ACU) of a tertiary hospital in Brasília, aimed to assess the quality of the service executed and suggest corrections if necessary. The methodology used was based on the National Health Surveillance Agency resolutions: n° 67/2007 and n° 220/2004, as well as the United States Pharmacopoeia (USP), considering two types of monitoring: environmental and operational. Environmental monitoring was performed with the identification of microorganisms in the air and surfaces, under operating conditions and at rest, using Petri dishes with nutrient agar; and operational monitoring was carried out based on the microbiological assessment of the pharmacist’s glove, which was authorized by the Human Ethics Committee of the University of Brasília. In both situations analyzed - in process and at rest there was a microbial count of 6.09 and 1.58 colony-forming units (CFU), respectively. As a result, it was found that the greater the number of people moving within the cleanroom and the number of drugs compounded during the process, the more difficult it is to maintain the conditions required by law. The identified microorganisms were common in the sterile compounding area, due to the presence of the pharmacist and the structural conditions of the cleanroom, hence the constant need for personnel training and adaptation of the structural conditions of the environment to better meet the requirements of the legislation.

DOI:https://doi.org/10.56238/devopinterscie-285