Hematological biomarkers in tilapia: Effect of residual concentrations of clove oil and florfenicol

Abstract

The use of clove oil as an anesthetic in fish management and florfenicol in the treatment of bacteriosis can leave residues in water causing damage to fish and environmental health. Hematological biomarkers are changes in blood parameters detected in organisms exposed to some agent or pollutant in water. Residues of clove oil and florfenicol on water may interfere with hematological biomarkers, impairing their interpretation during water quality monitoring. The objective of this study was to evaluate the effect of residual concentrations of clove oil and florfenicol on water on the response of hematological biomarkers in tilapia. The blood variables were evaluated in juvenile tilapias maintained for three and seven days in an aquarium system with a useful volume of 200 L without water recirculation.  Three treatments with three replications were used, each aquarium containing nine tilapias as a sampling unit. Experiment 1 was composed of the treatments: control (without diluent and without clove oil), clove oil at a concentration of 0.5mg/L with alcohol diluent at a concentration of 10μL/L and only alcohol diluent at a concentration of 10μL/L. Experiment 2 was composed of the treatments: 0.0 (control); 0.5 mg/L and 5 mg/L florfenicol. The blood variables collected during the two experiments showed no significant difference, as did the water monitoring parameters, such as pH, dissolved oxygen, temperature, conductivity, and total ammonia. Residual concentrations of clove oil and florfenicol in water do not interfere in the response of hematological biomarkers in tilapia.

DOI:https://doi.org/10.56238/sevened2023.004-080