Evaluation of the efficacy of the in vitro micronucleus method using a commercial kit and comparison with the microscopy method
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Abstract
In vitro micronucleus testing is an important tool in the investigation of the genotoxic potential of products. Performing the test by the optical microscopy method requires training and attention from the technician reading the slides and can be an extremely slow process. The technique of micronucleus analysis by flow cytometry emerges as a highly complex assay that makes it possible to increase the number of analyses and reduce the time spent on experiments. Some commercial kits are sold to perform this test, however, in some cases, they can be very costly for the laboratory. This work demonstrated that the technique of micronuclei in vitro by microscopy and cytometry are equivalent and that it is also possible to optimize the use of commercial reagents to reduce costs.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2023 Karine Renata Dias Silveira, Carla Lujan Villaroel, Hilana dos Santos Brunel, Rosângela Vieira de AndradeAuthor(s)
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Karine Renata Dias Silveira
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Hilana dos Santos Brunel
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Rosângela Vieira de Andrade